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KMID : 0358420080510080858
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Korean Journal of Obstetrics and Gynecology
2008 Volume.51 No. 8 p.858 ~ p.865
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Apoptosis of the mitochondria protein p32 (gc1qbp) in human ovarian cancer cells
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Won Mi-Ae
Lee Sun-Young
Ko Jeong-Jae
Bae Jee-Hyeon
Kim Seung-Jo
Lee Kang-Seok
Yoon Seong-Min
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Abstract
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Objective: The purpose of the study was to examine a possible physiological function of p32-mediated apoptosis signaling in ovarian cancer cells.
Methods: SK-OV-3 cells were transfected with respective plasmid DNAs, and cell viability was measured. By immunoprecipitation and immunofluorescence staining analysis, we confirmed that p32 interacts with Harakiri in ovarian cancer cells.
Results: In SK-OV-3 cells, p32 interacted with Harakiri and both p32 and Harakiri were colocalized in the mitochondria. In addition, overexpression of p32 induced apoptosis of ovarian cancer cells and augmented Harakiri-mediated apoptosis.
Conclusion: Our results demonstrated p32 as an apoptosis inducer and helped to provide the better understanding of the function of p32 in ovarian cancer cells and a possibility of p32 in the application of cancer therapeutics.
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KEYWORD
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p32, Harakiri, Apoptosis, Cell death, Ovarian cancer
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